Journal: Journal of Inflammation Research

Article Title: FLI1 Regulates Histamine Decarboxylase Expression to Control Inflammation Signaling and Leukemia Progression

doi: 10.2147/JIR.S401566

Figure Lengend Snippet: The HDC knockdown does not affect cell proliferation in culture. ( A and B ) Relative expression of HDC in shHDC1-3 cells by RT-qPCR ( A ) and Western blot ( B ). ( C ) Cell proliferation index in scrambled and shHDC2 cells. ( D and E ) Cell proliferation of HEL cells in culture following addition of the indicated concentrations of famotidine ( D ) and histamine ( E ). ( F ) Cell proliferation in shHDC2 and control cells with or without addition of histamine. ( G and H ) Relative expression of globin genes HBA1 ( G ) and HBA2 ( H ) in shHDC2 and control cells, by RT-qPCR. P=<0.01 (**) and P=<0.001 (***) by two-tailed Student’s t-test.

Article Snippet: Famotidine was purchased from (APExBIO), Tetrandrine from (Solarbio, CN), EGC from (Selleckchem, CN) and Diacerein from (APExBIO).

Techniques: Knockdown, Expressing, Quantitative RT-PCR, Western Blot, Control, Two Tailed Test

Journal: Journal of Inflammation Research

Article Title: FLI1 Regulates Histamine Decarboxylase Expression to Control Inflammation Signaling and Leukemia Progression

doi: 10.2147/JIR.S401566

Figure Lengend Snippet: Diacerein and tetrandrine inhibit leukemia progression in vivo. ( A – D ) Leukemic mice induced by F-MuLV were treated with 5 mg/kg histamine ( A ), 3 mg/kg famotidine ( B ), 3 mg/Kg diacerein ( C ) and 3 mg/Kg tetrandrine ( D ) for two weeks. Mice were observed for the development of leukemia and scored at the onset of death. The tumor growth was calculated by Student’s t-test and death was used to plot a Kaplan–Meier survival curve. ( E ) In this model, FLI1 controls HDC transcription, which itself regulates histamine production involved in allergenic reaction. HDC is also controls CXCR2 and IL1B associated with asthmatic reaction. These inflammatory factors likely effects leukemia progression through inflammation. While tetrandrine inhibits HDC through FLI1, EGC controls transcription of HDC through another mechanism.

Article Snippet: Famotidine was purchased from (APExBIO), Tetrandrine from (Solarbio, CN), EGC from (Selleckchem, CN) and Diacerein from (APExBIO).

Techniques: In Vivo